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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSMBIO
- 检测范围:
参照说明书
- 检测方法:
夹心法/竞争法
- 应用:
0.1ng/ml
- 适应物种:
人
- 标记物:
RAS guanyl- releasing protein 4, RASGRP4
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.31-20ng/ml
- 规格:
96T
Human RAS guanyl- releasing protein 4, RASGRP4 ELISA KIT
Product Name:Human RAS guanyl- releasing protein 4, RASGRP4 ELISA KIT
Packing:96T
Catalog No.:ELI-08500h
Gene Name:RASGRP4
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:RAS guanyl-releasing protein 4(RASGRP4
Alternative Name:RASGRP4,RAS guanyl-releasing protein 4(RASGRP4
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human RAS guanyl- releasing protein 4, RASGRP4 ELISA KIT allows for the in vitro quantitative determination of RAS guanyl-releasing protein 4(RASGRP4 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human RAS guanyl- releasing protein 4, RASGRP4 ELISA KIT has been pre-coated with an RAS guanyl-releasing protein 4(RASGRP4 antibody specific to RAS guanyl-releasing protein 4(RASGRP4 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for RAS guanyl-releasing protein 4(RASGRP4 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain RAS guanyl-releasing protein 4(RASGRP4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of RAS guanyl-releasing protein 4(RASGRP4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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