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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
参照说明书
- 检测方法:
夹心法/竞争法
- 应用:
0.1ng/ml
- 适应物种:
人
- 标记物:
Testis- specific H1 histone, H1FNT
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.31-20ng/ml
- 规格:
96T
Human Testis- specific H1 histone, H1FNT ELISA KIT
Product Name:Human Testis- specific H1 histone, H1FNT ELISA KIT
Packing:96T
Catalog No.:ELI-08480h
Gene Name:H1FNT
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:Testis-specific H1 histone(H1FNT
Alternative Name:H1FNT,Testis-specific H1 histone(H1FNT
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Testis- specific H1 histone, H1FNT ELISA KIT allows for the in vitro quantitative determination of Testis-specific H1 histone(H1FNT concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Testis- specific H1 histone, H1FNT ELISA KIT has been pre-coated with an Testis-specific H1 histone(H1FNT antibody specific to Testis-specific H1 histone(H1FNT .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Testis-specific H1 histone(H1FNT and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Testis-specific H1 histone(H1FNT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Testis-specific H1 histone(H1FNT in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验nM 5'biotin end-labeled siRNA under gentle agitation, i.e. a rocking plate (Figure 1a). We utilized the EF1 alpha specific siRNA, EF52, shown previously to direct TGS in human cells (Morris et al. 2004). The EF52 siRNA was constructed with a 5' biotin
your sequence for AA dinucleotides. A report is generated indicating the position of the AA dinucleotide, the 21 base target and the corresponding sense and antisense siRNA oligonucleotides. siRNA targets can then be sent directly to one of our kit-specific
的原理用于分子诊断方面:TrimGen公司的KRAS Mutation Detection Kit在96孔板里一次能实现7/8个KRAS突变位点的检测。 另外基于相同原理的技术还有PPT-ELISA。这是用来筛截短突变的,相关文章见:1,Rapid screen for truncating ATM mutations by PTT-ELISA . 2,An ELISA-based high throughput protein truncation test
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