- ￥2890
- LSM Bio
- 参照说明书
- 0.31-20ng/ml
- 国产
- ELI-08155h
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
参照说明书
- 检测方法：
夹心法/竞争法
- 应用：
0.1ng/ml
- 适应物种：
人
- 标记物：
GC- rich sequence DNA- binding factor 1, GCFC1
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.31-20ng/ml
- 规格：
96T
Human GC- rich sequence DNA- binding factor 1, GCFC1 ELISA KIT
Product Name:Human GC- rich sequence DNA- binding factor 1, GCFC1 ELISA KIT
Packing:96T
Catalog No.:ELI-08155h
Gene Name:GCFC1
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:GC-rich sequence DNA-binding factor 1(GCFC1
Alternative Name:GCFC1,GC-rich sequence DNA-binding factor 1(GCFC1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human GC- rich sequence DNA- binding factor 1, GCFC1 ELISA KIT allows for the in vitro quantitative determination of GC-rich sequence DNA-binding factor 1(GCFC1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human GC- rich sequence DNA- binding factor 1, GCFC1 ELISA KIT has been pre-coated with an GC-rich sequence DNA-binding factor 1(GCFC1 antibody specific to GC-rich sequence DNA-binding factor 1(GCFC1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for GC-rich sequence DNA-binding factor 1(GCFC1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain GC-rich sequence DNA-binding factor 1(GCFC1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of GC-rich sequence DNA-binding factor 1(GCFC1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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清楚，因此其需要后续进一步分析确定，此外，RLGS图谱不能完全确认所缺失的片段是由于甲基化所致还是由于DNA本身缺失所致[5]且结果分析复杂，不易解释。 2.3.2 MBD（Methyl-CpG binding domain column chromatography，甲基化结合区）柱层析法 根据MBD蛋白家族和MeCP2的特性，Masahiko Shiraishi等2004年[50]提出了一种新的方法MBD柱层析法，用于筛选和发现基因组中甲基化的情况。并且比较了MBD和重亚硫酸盐基因组测序法，得出：用MBD
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