- ￥2890
- LSM Bio
- 0.15-10ng/ml
- 0.078ng/ml
- 国产
- ELI-07558h
- 2025年07月12日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.15-10ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Cytochrome P450 2C19, CYP2C19
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078ng/ml
- 规格：
96T
Human Cytochrome P450 2C19, CYP2C19 ELISA KIT
Product Name:Human Cytochrome P450 2C19, CYP2C19 ELISA KIT
Packing:96T
Catalog No.:ELI-07558h
Gene Name:CYP2C19
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Cytochrome P450 2C19(CYP2C19
Alternative Name:CYP2C19,Cytochrome P450 2C19(CYP2C19
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Cytochrome P450 2C19, CYP2C19 ELISA KIT allows for the in vitro quantitative determination of Cytochrome P450 2C19(CYP2C19 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Cytochrome P450 2C19, CYP2C19 ELISA KIT has been pre-coated with an Cytochrome P450 2C19(CYP2C19 antibody specific to Cytochrome P450 2C19(CYP2C19 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Cytochrome P450 2C19(CYP2C19 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Cytochrome P450 2C19(CYP2C19, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Cytochrome P450 2C19(CYP2C19 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Evaluation of Cytochrome P450 Activities in Human Hepatocytes In Vitro
Major hepatic cytochrome P450 activities (CYP1A2, CYP2A6, CYP2B6, CYP2C9, CYP2C19, CYP2D6, CYP2E1, and CYP3A4) can be simultaneously examined in human hepatocytes by incubation with a cocktail of multiple specific probes. Cocktail strategy
Genotyping for Cytochrome P450 Polymorphisms
Protocols for the extraction of DNA from human blood and for genotyping for a number of common cytochrome P450 polymorphisms using either polymerase chain reaction (PCR)-restriction fragment length polymorphism or PCR-single-strand
Evaluation of Cytochrome P450 Inhibition in Human Liver Microsomes
Evaluation of lead compounds for P450 inhibition in human liver microsomes has been widely accepted as an in vitro approach to assess drug interaction potential. This chapter describes a detailed traditional CYP inhibition protocol
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