- ￥2890
- LSM Bio
- 0.15-10ng/ml
- 0.078ng/ml
- 国产
- ELI-07271h
- 2025年07月07日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.15-10ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Semaphorin- 3A, SEMA3A
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078ng/ml
- 规格：
96T
Human Semaphorin- 3A, SEMA3A ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
Semaphorin-3A,Semaphorin III(Sema III),
Search name
Human Semaphorin-3A ELISA Kit, Human SEMA3A ELISA Kit,Semaphorin-3A ELISA Kit,SEMA3A ELISA Kit,
Intended use
This immunoassay kit allows for the in vitro quantitative determination of human semaphorin-3a concentrations in serum, Plasma, Urine,
tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Semaphorin-3A. Standards or samples are then
added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Semaphorin-3A and
Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is
added to each well. Only those wells that contain Semaphorin-3A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a
change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is
measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Semaphorin-3A in the samples is then
determined by comparing the O.D. of the samples to the standard curve.
PDF manual download
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文献和实验上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 大鼠 Sema3A(Sema3A)ELISA 试剂盒 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗大鼠 Sema3A 单抗包被于酶标板上，标准品和样品中的 Sema3A 与单抗结合，加入生物素化的抗大鼠 Sema3A ，形成免疫复合物连接在板上，辣根过氧化物酶标记的 Streptavidin 与生物素结合
Warnings And Limitations This product is for research use only. Not intended for any animal or human therapeutic or diagnostic use unless otherwise stated. Follow appropriate laboratory guidelines. This product contains 0.02% sodium azide
RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
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