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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.15-10ng/ml
- 检测方法:
夹心法/竞争法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Telomerase reverse transcriptase, TERT
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078ng/ml
- 规格:
96T
Human Telomerase reverse transcriptase, TERT ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
TERT,CMM9; DKCA2; DKCB4; EST2; PFBMFT1; TCS1; TP2; TRT; hEST2; hTRT; telomerase catalytic subunit; telomerase-associated protein 2; telomerase reverse transcriptase,Telomerase reverse transcriptase,Telomerase-associated protein 2,TP2,HEST2,Telomerase catalytic subunit,
Search name
Human TERT ELISA KIT ,Human CMM9 ELISA KIT ,Human DKCA2 ELISA KIT ,Human DKCB4 ELISA KIT ,Human EST2 ELISA KIT ,Human PFBMFT1 ELISA KIT ,Human TCS1 ELISA KIT ,Human TP2 ELISA KIT ,Human TRT ELISA KIT ,Human hEST2 ELISA KIT ,Human hTRT ELISA KIT ,Human telomerase catalytic subunit ELISA KIT ,Human telomerase-associated protein 2 ELISA KIT ,Human telomerase reverse transcriptase ELISA KIT ,Human Telomerase reverse transcriptase ELISA KIT ,Human Telomerase-associated protein 2 ELISA KIT ,Human TP2 ELISA KIT ,Human HEST2 ELISA KIT ,Human Telomerase catalytic subunit ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of human Telomerase reverse transcriptase concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Telomerase reverse transcriptase. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Telomerase reverse transcriptase and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Telomerase reverse transcriptase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Telomerase reverse transcriptase in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PDF Manual download
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文献和实验a reusable pool of first-strand cDNA coupled to a solid phase. Nucleic Acids Res. 19 : 4010-20 Rodriguez, I.R., Mazuruk, K.,Schoen, T.J. and Chader, J.G. (1994). Structural analysis of the human hydroxyindole- O-methyltransferase gene : presence
and trans -ligates an exon tagged at its 3ʹ end onto the downstream U nucleotide of the targeted RNA. Here, we describe the specific trans -splicing ribozyme that can sense and reprogram human telomerase reverse transcriptase (hTERT)-encoding RNA
by creating a reusable pool of first-strand cDNA coupled to a solid phase. Nucleic Acids Res. 19 : 4010-20 Rodriguez, I.R., Mazuruk, K.,Schoen, T.J. and Chader, J.G. (1994). Structural analysis of the human hydroxyindole- O-methyltransferase gene : presence
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