- ￥2890
- LSM Bio
- 0.15-10ng/ml
- 0.078ng/ml
- 国产
- ELI-06488h
- 2025年07月07日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.15-10ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Secretory phospholipase A2 receptor, PLA2R1
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078ng/ml
- 规格：
96T
Human Secretory phospholipase A2 receptor, PLA2R1 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Secretory phospholipase A2 receptor,PLA2-R,PLA2R, CLEC13C ,M-type receptor,180 kDa secretory phospholipase A2 receptor,C-type lectin domain family 13 member C, PLA2R1,PLA2R; CLEC13C; PLA2-R; PLA2G1R; PLA2IR; 180 kDa secretory phospholipase A2 receptor; phospholipase A2 receptor 1 180kDa, secretory phospholipase A2 receptor 180 kDa
Search name
Human Secretory phospholipase A2 receptor ELISA KIT ,Human PLA2-R ELISA KIT ,Human PLA2R ELISA KIT ,Human M-type receptor ELISA KIT ,Human 180 kDa secretory phospholipase A2 receptor ELISA KIT ,Human C-type lectin domain family 13 member C ELISA KIT ,Human PLA2R1 ELISA KIT ,Human PLA2R ELISA KIT ,Human CLEC13C ELISA KIT ,Human PLA2-R ELISA KIT ,Human PLA2G1R ELISA KIT ,Human PLA2IR ELISA KIT ,Human 180 kDa secretory phospholipase A2 receptor ELISA KIT ,Human phospholipase A2 receptor 1 180kDa ELISA KIT ,Human secretory phospholipase A2 receptor 180 kDa ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of human secretory phospholipase a2 receptor,PLA2R concentrations in serum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to PLA2R. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for PLA2R and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain PLA2R, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PLA2R in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PDF Manual download
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文献和实验Purification and Assay of Mammalian Group I and Group IIa Secretory Phospholipase A2
Phospholipases A2 (PLA2 ) are a family of ubiquitous lipolytic enzymes that are found both as intracellular and secreted proteins. Secretory PLA2 s are small (14 kDa), homologous proteins that require millimolar Ca2+ for catalytic activity
Synchronizing Protein Transport in the Secretory Pathway
or transported to their target compartments, newly synthesized proteins leave the endoplasmic reticulum to reach the Golgi apparatus, where they are processed and sorted toward their final destinations along the secretory pathway. It is now clear that many Golgi
Introduction of human 14-3-3 proteins
whose members have been shown to activate tyrosine and tryptophan hydroxylases,inhibit protein kinase C and possess phospholipase A2 activity in vitro.We have isolated and analyzed a 14-3-3 protein cDNA clone (H14-3-3)from a human fetal brain cDNA library
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