- ￥2890
- LSM Bio
- 0.15-10ng/ml
- 0.078ng/ml
- 国产
- ELI-06055h
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.15-10ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Cyclin- dependent kinase 1, CDK1
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078ng/ml
- 规格：
96T
Human Cyclin- dependent kinase 1, Cdk1 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
CDC2,cell cycle controller CDC2; CDC28A; P34CDC2; cell division control protein 2 homolog; cell division cycle 2, G1 to S and G2 to M; cell division protein kinase 1; p34 protein kinase;CDK1,CDC2;CDC28A;CDKN1;P34CDC2,Cyclin-dependent kinase 1,CDK1, p34 protein kinase;Cell division control protein 2 homolog;Cyclin-dependent kinase inhibitor 1,p21,CDK-interacting protein 1,Melanoma differentiation-associated protein,
Search name
Human CDC2 ELISA KIT ,Human cell cycle controller CDC2 ELISA KIT ,Human CDC28A ELISA KIT ,Human P34CDC2 ELISA KIT ,Human cell division control protein 2 homolog ELISA KIT ,Human cell division cycle 2 ELISA KIT ,Human G1 to S and G2 to M ELISA KIT ,Human cell division protein kinase 1 ELISA KIT ,Human p34 protein kinase;CDK1 ELISA KIT ,Human CDC2;CDC28A;CDKN1;P34CDC2 ELISA KIT ,Human Cyclin-dependent kinase 1 ELISA KIT ,Human CDK1 ELISA KIT ,Human p34 protein kinase;Cell division control protein 2 homolog;Cyclin-dependent kinase inhibitor 1 ELISA KIT ,Human p21 ELISA KIT ,Human CDK-interacting protein 1 ELISA KIT ,Human Melanoma differentiation-associated protein ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Human cyclin-dependent kinase inhibitor 1,Cyclin-dependent kinase inhibitor 1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cyclin-dependent kinase inhibitor 1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Cyclin-dependent kinase inhibitor 1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Cyclin-dependent kinase inhibitor 1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Cyclin-dependent kinase inhibitor 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Assays for Cyclin-Dependent Kinase Inhibitors
Cyclin-dependent kinases (CDK) are serine/threonine kinases that regulate cell cycle control and progression (1 ,2 ). A CDK holoenzyme complex is active if associated with its cyclin partner and if the complex is phosphorylated at specific
Analysis of Cyclin-Dependent Kinase Activity
are therefore needed to test chemical candidates for such therapy. Here we present a protocol for analysis of kinase activity for cyclin-dependent kinases using general substrates that will detect the broad activity of cyclin-dependent kinases (Fig. 1 ).
Detection of DNA-Dependent Protein Kinase in Extracts from Human and Rodent Cells
The DNA-dependent protein kinase, DNA-PK, is required for DNA double-strand break repair and V(D)J recombination (1 –3 ). DNA-PK is composed of a large catalytic subunit of approx 460 kDa (DNA-PKcs) and a heterodimeric DNA targeting subunit
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