- ￥2890
- LSM Bio
- 0.15-10ng/ml
- 0.078ng/ml
- 国产
- ELI-05899h
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.15-10ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Sonic hedgehog protein, SHH
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078ng/ml
- 规格：
96T
Human Sonic hedgehog protein, SHH ELISA KIT
Product Name:Human Sonic hedgehog protein, SHH ELISA KIT
Packing:96T
Catalog No.:ELI-05899h
Gene Name:SHH
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:Sonic hedgehog protein(SHH
Alternative Name:SHH,Sonic hedgehog protein(SHH
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Sonic hedgehog protein, SHH ELISA KIT allows for the in vitro quantitative determination of Sonic hedgehog protein(SHH concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Sonic hedgehog protein, SHH ELISA KIT has been pre-coated with an Sonic hedgehog protein(SHH antibody specific to Sonic hedgehog protein(SHH .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Sonic hedgehog protein(SHH and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Sonic hedgehog protein(SHH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Sonic hedgehog protein(SHH in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验人Sonic Hedgehog(SHH)ELISA试剂盒 说明书
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 人 Sonic Hedgehog ( SHH )ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 SHH 单抗包被于酶标板上，标准品和样品中的 SHH与单抗结合，加入生物
Application of Sonic Hedgehog to the Developing Chick Limb
Here, we describe methods for applying Sonic hedgehog (Shh) to developing chick limbs. The Sonic hedgehog gene is expressed in the polarizing region, a signaling region at the posterior margin of the limb bud and application of Shh-expressing
SYendocrine 想请问各位大虾，sonic hedgehog通路的特异性激活剂是什么？ （附加：另外有没有哪位知道该通路在肾小球系膜细胞中是否表达，如果想测是否表达通过RT-PCR测SHH，通过Elisa法测shh蛋白就行了么？） 请各位大虾不吝赐教！！ 大鹏鸟 好像只有SHH能激活，没看到有文献说有其他激活剂的。 想测表达与否还是通过传统的方法,RT-PCR ,WESTERN，ELISA
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