Human Cell division cycle protein 23 homolog, CDC23 ELISA KIT
Product Name:Human Cell division cycle protein 23 homolog, CDC23 ELISA KIT
Packing:96T
Catalog No.:ELI-05747h
Gene Name:CDC23
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Cell division cycle protein 23 homolog(CDC23
Alternative Name:CDC23,Cell division cycle protein 23 homolog(CDC23
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Cell division cycle protein 23 homolog, CDC23 ELISA KIT allows for the in vitro quantitative determination of Cell division cycle protein 23 homolog(CDC23 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Cell division cycle protein 23 homolog, CDC23 ELISA KIT has been pre-coated with an Cell division cycle protein 23 homolog(CDC23 antibody specific to Cell division cycle protein 23 homolog(CDC23 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Cell division cycle protein 23 homolog(CDC23 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Cell division cycle protein 23 homolog(CDC23, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Cell division cycle protein 23 homolog(CDC23 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!