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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.15-10ng/ml
- 检测方法:
夹心法/竞争法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Casein kinase II subunit beta, CSNK2B
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078ng/ml
- 规格:
96T
Human Casein kinase II subunit beta, CSNK2B ELISA KIT
Product Name:Human Casein kinase II subunit beta, CSNK2B ELISA KIT
Packing:96T
Catalog No.:ELI-05492h
Gene Name:CSNK2B
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:Casein kinase II subunit beta(CSNK2B
Alternative Name:CSNK2B,Casein kinase II subunit beta(CSNK2B
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Casein kinase II subunit beta, CSNK2B ELISA KIT allows for the in vitro quantitative determination of Casein kinase II subunit beta(CSNK2B concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Casein kinase II subunit beta, CSNK2B ELISA KIT has been pre-coated with an Casein kinase II subunit beta(CSNK2B antibody specific to Casein kinase II subunit beta(CSNK2B .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Casein kinase II subunit beta(CSNK2B and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Casein kinase II subunit beta(CSNK2B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Casein kinase II subunit beta(CSNK2B in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验(3 ). PKA is composed of two catalytic (C) and two regulatory (R) subunits and has type I and type II isozymes, with different R subunits, termed RI and RII, interacting with an identical C subunit (1 ). Thus far, four isoforms of R subunits, RIα, RIβ, RIIα
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
., Graves, D.J., DeMaggio, A.J., Hoekstra, M.F., Blenis, J., Hunter, T., and Cantley, L.C. 1996. A structural basis for substrate specificities of protein Ser/Thr kinases: Primary sequence preference of. casein kinases I and II, NIMA, phosphorylase kinase
Introduction of human 14-3-3 proteins
whose members have been shown to activate tyrosine and tryptophan hydroxylases,inhibit protein kinase C and possess phospholipase A2 activity in vitro.We have isolated and analyzed a 14-3-3 protein cDNA clone (H14-3-3)from a human fetal brain cDNA library
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