- ￥2890
- LSM Bio
- 0.15-10ng/ml
- 0.078ng/ml
- 国产(香港）
- ELI-05409h
- 2026年04月29日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
20
- 供应商：
LSMBIO
- 检测范围：
0.15-10ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
C- C motif chemokine 27, CCL27
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078ng/ml
- 规格：
96T
Human C- C motif chemokine 27, CCL27 ELISA KIT
Product Name:Human C- C motif chemokine 27, CCL27 ELISA KIT
Packing:96T
Catalog No.:ELI-05409h
Gene Name:CCL27
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:C-C motif chemokine 27(CCL27
Alternative Name:CCL27,C-C motif chemokine 27(CCL27
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human C- C motif chemokine 27, CCL27 ELISA KIT allows for the in vitro quantitative determination of C-C motif chemokine 27(CCL27 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human C- C motif chemokine 27, CCL27 ELISA KIT has been pre-coated with an C-C motif chemokine 27(CCL27 antibody specific to C-C motif chemokine 27(CCL27 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for C-C motif chemokine 27(CCL27 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain C-C motif chemokine 27(CCL27, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of C-C motif chemokine 27(CCL27 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Active Motif ChIP 实验专家技术经验分享：如何判断 ChIP 成功与否？
region) 253233C (Positive region)253123表 1，CT 值汇总根据上面的公式推导出富集度如下表格所示：EnrichmentIgGH3K4me3A (Target region)5%×2(26-32)≈0.08%5%×2(26-27)=2.5%B (Negative region)5%×2(25-32)≈0.04%5%×2(25-33)≈0.02%C (Positive region)5%×2(25-31)≈0.08%5%×2(25-23)=20%表 2，富集度汇总
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
in many signaling pathways typically associated with human malignancies. Phospho?motif antibodies are engineered to recognize proteins that contain a phosphorylated residue in the context of a specific motif. They are raised against a library of phospho?peptides
Bianchi, E., Steinkuhler, C., Taliani, M., Urbani, A., Francesco, R.D., and Pessi, A. 1996. Synthetic depsipeptide substrates for the assay of human hepatitis C virus protease. Anal. Biochem. 237:239‐244
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