- ￥2890
- LSM Bio
- 0.15-10ng/ml
- 0.078ng/ml
- 国产(香港）
- ELI-05195h
- 2026年05月21日
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- 文献和实验
- 技术资料
- 库存：
20
- 供应商：
LSMBIO
- 检测范围：
0.15-10ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Cytosolic beta- glucosidase, GBA3
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078ng/ml
- 规格：
96T
Human Cytosolic beta glucosidase, GBA3 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Class 3.2.1.21,Cytosolic beta-glucosidases,Cytosolic beta-glucosidase-like protein 1, GBA3,CBG,CBGL1,GLUC,KLRP,cytosolic beta-glucosidase,cytosolic beta-glucosidase-like protein 1,glucosidase, beta, acid 3 (cytosolic),klotho-related protein,glucosidase beta acid 3
Search name
Human Class 3.2.1.21 ELISA KIT ,Human Cytosolic beta-glucosidases ELISA KIT ,Human Cytosolic beta-glucosidase-like protein 1 ELISA KIT ,Human GBA3 ELISA KIT ,Human CBG ELISA KIT ,Human CBGL1 ELISA KIT ,Human GLUC ELISA KIT ,Human KLRP ELISA KIT ,Human cytosolic beta-glucosidase ELISA KIT ,Human cytosolic beta-glucosidase-like protein 1 ELISA KIT ,Human glucosidase ELISA KIT ,Human beta ELISA KIT ,Human acid 3 (cytosolic) ELISA KIT ,Human klotho-related protein ELISA KIT ,Human glucosidase beta acid 3 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of human cytosolic beta-glucosidase,Cytosolic beta-glucosidase concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytosolic beta-glucosidase. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Cytosolic beta-glucosidase and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Cytosolic beta-glucosidase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Cytosolic beta-glucosidase in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PDF Manual download
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文献和实验调至 1μg/μl。 4. ELISA 检测：按试剂盒说明书完成板平衡、加样孵育、洗板、酶标抗体孵育、显色终止与读数，结合标准曲线得出 IFN-γ 浓度。 四、操作要点速记 1.组织样本新鲜处理，全程低温操作，裂解液现配现用； 2. 蛋白定量时标准品与样本同步孵育，稀释采用倍比稀释减少误差； 3. ELISA 实验需每次重绘标准曲线，洗板充分、严格把控显色与终止时间。 免疫检测试剂盒： 货号-规格 名称 abs510001-96T Human IL-2 ELISA Kit (Faster
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