- ￥2890
- LSM Bio
- 0.15-10ng/ml
- 0.078ng/ml
- 国产
- ELI-05105h
- 2025年07月12日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.15-10ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
V(D)J recombination- activating protein 1, RAG1
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078ng/ml
- 规格：
96T
Human V(D)J recombination- activating protein 1, RAG1 ELISA KIT
Product Name:Human V(D)J recombination- activating protein 1, RAG1 ELISA KIT
Packing:96T
Catalog No.:ELI-05105h
Gene Name:RAG1
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:V(D)J recombination-activating protein 1(RAG1
Alternative Name:RAG1,V(D)J recombination-activating protein 1(RAG1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human V(D)J recombination- activating protein 1, RAG1 ELISA KIT allows for the in vitro quantitative determination of V(D)J recombination-activating protein 1(RAG1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human V(D)J recombination- activating protein 1, RAG1 ELISA KIT has been pre-coated with an V(D)J recombination-activating protein 1(RAG1 antibody specific to V(D)J recombination-activating protein 1(RAG1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for V(D)J recombination-activating protein 1(RAG1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain V(D)J recombination-activating protein 1(RAG1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of V(D)J recombination-activating protein 1(RAG1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验., Satomi, Y., Kurata, T., Ueno, N.,Norioka, S., Kondoh, H., et al. (2006)Monounsaturated fatty acid modificationof Wnt protein: its role in Wnt secretion. Dev Cell , 11 , 791-801. 3. Willert, K., Brown, J. D., Danenberg, E., Duncan, A. W., Weissman, I. L
) Lithium inhibits caspase 3 activation and dephosphorylation of PKB and GSK3 induced by K+ deprivation in cerebellar granule cells. J Neurochem. 78 , 199–206. 18. Ilouz R, Kaidanovich O, Gurwitz D, Eldar-Finkelman H. (2002) Inhibition
Detection of DNA-Dependent Protein Kinase in Extracts from Human and Rodent Cells
The DNA-dependent protein kinase, DNA-PK, is required for DNA double-strand break repair and V(D)J recombination (1 –3 ). DNA-PK is composed of a large catalytic subunit of approx 460 kDa (DNA-PKcs) and a heterodimeric DNA targeting subunit
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