- ￥2890
- LSM Bio
- 0.15-10ng/ml
- 0.078ng/ml
- 国产
- ELI-04649h
- 2025年07月10日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.15-10ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Carbonic anhydrase 9, CA9
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078ng/ml
- 规格：
96T
Human Carbonic anhydrase 9, CA9 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Carbonic anhydrase 9,pMW1,Renal cell carcinoma-associated antigen G250,RCC-associated antigen G250,P54/58N,Membrane antigen MN,Carbonate dehydratase IX,Carbonic anhydrase IX,CA-IX,CAIX, CA9, P54/58N,RCC-associated protein G250,carbonate dehydratase IX,carbonic dehydratase,membrane antigen MN,pMW1,renal cell carcinoma-associated antigen G250,carbonic anhydrase IX
Search name
Human Carbonic anhydrase 9 ELISA KIT ,Human pMW1 ELISA KIT ,Human Renal cell carcinoma-associated antigen G250 ELISA KIT ,Human RCC-associated antigen G250 ELISA KIT ,Human P54/58N ELISA KIT ,Human Membrane antigen MN ELISA KIT ,Human Carbonate dehydratase IX ELISA KIT ,Human Carbonic anhydrase IX ELISA KIT ,Human CA-IX ELISA KIT ,Human CAIX ELISA KIT ,Human CA9 ELISA KIT ,Human P54/58N ELISA KIT ,Human RCC-associated protein G250 ELISA KIT ,Human carbonate dehydratase IX ELISA KIT ,Human carbonic dehydratase ELISA KIT ,Human membrane antigen MN ELISA KIT ,Human pMW1 ELISA KIT ,Human renal cell carcinoma-associated antigen G250 ELISA KIT ,Human carbonic anhydrase IX ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of human Carbonic anhydrase 9 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Carbonic anhydrase 9. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Carbonic anhydrase 9 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Carbonic anhydrase 9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Carbonic anhydrase 9 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PDF Manual download
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文献和实验【Jounal club】NATURE MEDICINE: 利用蛋白组技术发现糖尿病眼底病变新机制
spectroscopy–based proteomics, we detected 117 proteins in human vitreous and elevated levels of extracellular carbonic anhydrase-I (CA-I) in vitreous from individuals with diabetic retinopathy, suggesting that retinal hemorrhage and erythrocyte lysis
The Application of the Chemical Array for Biological Study
library GLORIA (Gene Library of Osada Laboratory at RIKEN for chemical array analysis) has been established. Using the systematic platform, we detected novel inhibitors of carbonic anhydrase II. We also have shown that this screening method is useful
有替代前者的趋势。由于ABS-ELISA较普通ELISA多用了两种试剂，增加了操作步骤，在临床检验中ABS-ELISA应用不多。科研项目中检测微量的成分如细胞因子常采用本法。晶美分装ELISA KIT采用的方法：1， TORCH及传染病试剂盒（间接法），见2.2.32， TORCH-IgM捕获法特色：包被抗体，标记抗原原理：3， 细胞因子试剂盒采用的方法路线（ABC-ELISA）原理产品特色：采用ABC法，灵敏度更高，特异性更强。生物素抗体和酶联物是浓缩的，使用前需用相应的缓冲液稀释。酶联物可以通用
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