- ￥2890
- LSM Bio
- 0.15-10ng/ml
- 0.078ng/ml
- 国产
- ELI-04593h
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.15-10ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Glutamate- cysteine ligase catalytic subunit, GCLC ELISA K
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078ng/ml
- 规格：
96T
Human Glutamate- cysteine ligase catalytic subunit, GCLC ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
GCLC,GCL; GCS; GLCL; GLCLC; GCS heavy chain; gamma-ECS; gamma-glutamylcysteine synthetase; glutamate--cysteine ligase catalytic subunit; glutamate-cysteine ligase, catalytic subunit,Glutamate--cysteine ligase catalytic subunit),Gamma-glutamylcysteine synthetase,GCS heavy chain,Gamma-ECS,
Search name
Human GCLC ELISA KIT ,Human GCL ELISA KIT ,Human GCS ELISA KIT ,Human GLCL ELISA KIT ,Human GLCLC ELISA KIT ,Human GCS heavy chain ELISA KIT ,Human gamma-ECS ELISA KIT ,Human gamma-glutamylcysteine synthetase ELISA KIT ,Human glutamate--cysteine ligase catalytic subunit ELISA KIT ,Human glutamate-cysteine ligase catalytic subunit ELISA KIT ,Human Glutamate--cysteine ligase catalytic subunit ELISA KIT ,Human Gamma-glutamylcysteine synthetase ELISA KIT ,Human GCS heavy chain ELISA KIT ,Human Gamma-ECS ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of human glutamate--cysteine ligase catalytic subunit,Glutamate--cysteine ligase catalytic subunit concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutamate--cysteine ligase catalytic subunit. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Glutamate--cysteine ligase catalytic subunit and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Glutamate--cysteine ligase catalytic subunit, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Glutamate--cysteine ligase catalytic subunit in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PDF Manual download
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