Human Sperm protamine P1,PRM1

Human Sperm protamine P1, PRM1 ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Sperm protamine P1,Cysteine-rich protamine, PRM1,sperm protamine P1; CT94.1; cancer/testis antigen family 94, member 1; cysteine-rich protamine; testis specific protamine 1; protamine 1

Search name

Human Sperm protamine P1 ELISA KIT ,Human Cysteine-rich protamine ELISA KIT ,Human PRM1 ELISA KIT ,Human sperm protamine P1 ELISA KIT ,Human CT94.1 ELISA KIT ,Human cancer/testis antigen family 94 ELISA KIT ,Human  member 1 ELISA KIT ,Human cysteine-rich protamine ELISA KIT ,Human testis specific protamine 1 ELISA KIT ,Human protamine 1 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of human sperm protamine p1,Sperm protamine P1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sperm protamine P1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Sperm protamine P1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Sperm protamine P1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Sperm protamine P1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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