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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.15-10ng/ml
- 检测方法:
夹心法/竞争法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Mitogen- activated protein kinase 3, MAPK3
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078ng/ml
- 规格:
96T
Human Mitogen activated protein kinase 3,MAP kinase 3, MAPK 3 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Mitogen-activated protein kinase 3,MAP kinase 3,MAPK 3,Mitogen-activated protein kinase 1,MAP kinase 1,MAPK 1,Microtubule-associated protein 2 kinase,MAP kinase isoform p44,p44-MAPK,Insulin-stimulated MAP2 kinase,p44-ERK1,ERT2,Extracellular signal-regulated kinase 1,ERK1,ERK-1; ERT2; HS44KDAP; HUMKER1A; P44ERK1; P44MAPK; PRKM3; p44-ERK1; p44-MAPK; MAP kinase isoform p44; MAPK 1; extracellular signal-regulated kinase 1; extracellular signal-related kinase 1; insulin-stimulated MAP2 kinase; microtubule-associated protein 2 kinase; mitogen-activated protein kinase 3
Search name
Human Mitogen-activated protein kinase 3 ELISA KIT ,Human MAP kinase 3 ELISA KIT ,Human MAPK 3 ELISA KIT ,Human Mitogen-activated protein kinase 1 ELISA KIT ,Human MAP kinase 1 ELISA KIT ,Human MAPK 1 ELISA KIT ,Human Microtubule-associated protein 2 kinase ELISA KIT ,Human MAP kinase isoform p44 ELISA KIT ,Human p44-MAPK ELISA KIT ,Human Insulin-stimulated MAP2 kinase ELISA KIT ,Human p44-ERK1 ELISA KIT ,Human ERT2 ELISA KIT ,Human Extracellular signal-regulated kinase 1 ELISA KIT ,Human ERK1 ELISA KIT ,Human ERK-1 ELISA KIT ,Human ERT2 ELISA KIT ,Human HS44KDAP ELISA KIT ,Human HUMKER1A ELISA KIT ,Human P44ERK1 ELISA KIT ,Human P44MAPK ELISA KIT ,Human PRKM3 ELISA KIT ,Human p44-ERK1 ELISA KIT ,Human p44-MAPK ELISA KIT ,Human MAP kinase isoform p44 ELISA KIT ,Human MAPK 1 ELISA KIT ,Human extracellular signal-regulated kinase 1 ELISA KIT ,Human extracellular signal-related kinase 1 ELISA KIT ,Human insulin-stimulated MAP2 kinase ELISA KIT ,Human microtubule-associated protein 2 kinase ELISA KIT ,Human mitogen-activated protein kinase 3 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of human mitogen-activated protein kinase 3,MAP kinase 3 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to MAP kinase 3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for MAP kinase 3 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain MAP kinase 3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of MAP kinase 3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PDF Manual download
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文献和实验Analysis of Mitogen-Activated Protein Kinase Activity in Yeast
Mitogen-activated protein (MAP) kinases play central roles in transmitting extracellular and intracellular information in a wide variety of situations in eukaryotic cells. Their activities are perturbed in a large number of diseases
Mitogen-Activated Protein Kinase Signaling in Drug-Resistant Neuroblastoma Cells
with altered response to growth factor signals. The ubiquitous mitogen-activated protein kinase (MAPk) cascade, which transmits growth factor signals from the cell membrane to the nucleus, provides a principal mechanism for regulation of cell cycle progression
Detection of DNA-Dependent Protein Kinase in Extracts from Human and Rodent Cells
The DNA-dependent protein kinase, DNA-PK, is required for DNA double-strand break repair and V(D)J recombination (1 –3 ). DNA-PK is composed of a large catalytic subunit of approx 460 kDa (DNA-PKcs) and a heterodimeric DNA targeting subunit
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