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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.15-10ng/ml
- 检测方法:
夹心法/竞争法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Arachidonate 15- lipoxygenase B, ALOX15B
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078ng/ml
- 规格:
96T
Human Arachidonate 15- lipoxygenase B, ALOX15B ELISA KIT
Product Name:Human Arachidonate 15- lipoxygenase B, ALOX15B ELISA KIT
Packing:96T
Catalog No.:ELI-04468h
Gene Name:ALOX15B
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Arachidonate 15-lipoxygenase B(ALOX15B
Alternative Name:ALOX15B,Arachidonate 15-lipoxygenase B(ALOX15B
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Arachidonate 15- lipoxygenase B, ALOX15B ELISA KIT allows for the in vitro quantitative determination of Arachidonate 15-lipoxygenase B(ALOX15B concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Arachidonate 15- lipoxygenase B, ALOX15B ELISA KIT has been pre-coated with an Arachidonate 15-lipoxygenase B(ALOX15B antibody specific to Arachidonate 15-lipoxygenase B(ALOX15B .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Arachidonate 15-lipoxygenase B(ALOX15B and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Arachidonate 15-lipoxygenase B(ALOX15B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Arachidonate 15-lipoxygenase B(ALOX15B in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验samples, MPC-15 for 1–15 ml samples and MPC-50 for 15–50 ml samples. Mixer allowing both tilting and rotation. Buffer 1: PBS (without Ca2 and Mg2 ) w/0.1% BSA and 2 mM EDTA, pH 7.4. Buffer 2: PBS pH 7.4 (without Ca2 and Mg2 ).
A simple workflow allows the purification of high-quality DNA and RNA from the same sample (see flowchart). The 96-well purification plates of the kit are rapidly and conveniently processed using either a centrifuge (Centrifuge 4-15C and Plate Rotor 2 x 96
RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
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