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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.15-10ng/ml
- 检测方法:
夹心法/竞争法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Apoptosis regulator BAX, BAX
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078ng/ml
- 规格:
96T
Human Apoptosis regulator BAX, BAX ELISA KIT
Product Name:Human Apoptosis regulator BAX, BAX ELISA KIT
Packing:96T
Catalog No.:ELI-04433h
Gene Name:BAX
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:Apoptosis regulator BAX(BAX
Alternative Name:BAX,Apoptosis regulator BAX(BAX
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Apoptosis regulator BAX, BAX ELISA KIT allows for the in vitro quantitative determination of Apoptosis regulator BAX(BAX concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Apoptosis regulator BAX, BAX ELISA KIT has been pre-coated with an Apoptosis regulator BAX(BAX antibody specific to Apoptosis regulator BAX(BAX .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Apoptosis regulator BAX(BAX and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Apoptosis regulator BAX(BAX, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Apoptosis regulator BAX(BAX in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Molecular Characterization of the Proapoptotic Protein Bax
by immunoprecipitation (1 ). Subsequently it was shown that overexpression of Bax can accelerate cell death in response to various apoptosis stimuli (2 ). Physiologically, Bax plays an important role in neuronal development and spermatogenesis. Animals
Differential Expression of Apoptosis-Associated Genes bax and bcl-2 in Ovarian Cancer
either as inhibitors (e.g., bcl-2, bcl-xl, mcl- 1) or promoters (e.g., bcl-xs, bax, bak, bad) of apoptosis (1 -3 ). The chromosomal translocation t(14;18), leading to overexpression of the Bcl-2 protein was first described in human B-cell lymphoma (4 ). Later
-1640/10%FCS Anti-human CD95, clone EOS9.1 (eBioscience Cat. No. 16-0958) 24-well culture plate (Costar Cat. No. 3526) 12x75 mm flow cytometry centrifuge tubes (Falcon Cat. No. 2008) Apoptosis detection kit (eBioscience Apo-Direct
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