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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.15-10ng/ml
- 检测方法:
夹心法/竞争法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Epithelial cell adhesion molecule, EPCAM
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078ng/ml
- 规格:
96T
Human Epithelial cell adhesion molecule, EPCAM ELISA KIT
Product Name:Human Epithelial cell adhesion molecule, EPCAM ELISA KIT
Packing:96T
Catalog No.:ELI-04243h
Gene Name:EPCAM
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Epithelial cell adhesion molecule(EPCAM
Alternative Name:EPCAM,Epithelial cell adhesion molecule(EPCAM
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Epithelial cell adhesion molecule, EPCAM ELISA KIT allows for the in vitro quantitative determination of Epithelial cell adhesion molecule(EPCAM concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Epithelial cell adhesion molecule, EPCAM ELISA KIT has been pre-coated with an Epithelial cell adhesion molecule(EPCAM antibody specific to Epithelial cell adhesion molecule(EPCAM .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Epithelial cell adhesion molecule(EPCAM and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Epithelial cell adhesion molecule(EPCAM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Epithelial cell adhesion molecule(EPCAM in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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