- ￥2890
- LSM Bio
- 0.15-10ng/ml
- 0.078ng/ml
- 国产(香港）
- ELI-04100h
- 2026年05月21日
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- 文献和实验
- 技术资料
- 库存：
20
- 供应商：
LSMBIO
- 检测范围：
0.15-10ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Fibrinogen alpha chain, FGA
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078ng/ml
- 规格：
96T
Human Fibrinogen alpha chain, FGA ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Fibrinogen alpha chain, FAP,Fibroblast activation protein,FGA, fibroblast activation protein alpha; DPPIV; FAPA; 170 kDa melanoma membrane-bound gelatinase; integral membrane serine protease; seprase
Search name
Human Fibrinogen alpha chain ELISA KIT ,Human FAP ELISA KIT ,Human Fibroblast activation protein ELISA KIT ,Human FGA ELISA KIT ,Human fibroblast activation protein alpha ELISA KIT ,Human DPPIV ELISA KIT ,Human FAPA ELISA KIT ,Human 170 kDa melanoma membrane-bound gelatinase ELISA KIT ,Human integral membrane serine protease ELISA KIT ,Human seprase ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of human fibrinogen alpha chain,FGA concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to FGA. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for FGA and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain FGA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of FGA in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PDF Manual download
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文献和实验Assay (Invitrogen) as suggested by Illumina. Illumina Bead Array MicroRNA Detection Starting with 500 ng/sample of total RNA, mature microRNAs were amplified with the Illumina human v1 MicroRNA expression profiling kit containing primers for 743
调至 1μg/μl。 4. ELISA 检测：按试剂盒说明书完成板平衡、加样孵育、洗板、酶标抗体孵育、显色终止与读数，结合标准曲线得出 IFN-γ 浓度。 四、操作要点速记 1.组织样本新鲜处理，全程低温操作，裂解液现配现用； 2. 蛋白定量时标准品与样本同步孵育，稀释采用倍比稀释减少误差； 3. ELISA 实验需每次重绘标准曲线，洗板充分、严格把控显色与终止时间。 免疫检测试剂盒： 货号-规格 名称 abs510001-96T Human IL-2 ELISA Kit (Faster
Introduction of human 14-3-3 proteins
regulation.We report here the crystal structure of the human T-cell 14-3-3 isoform (tau)dimer at 2.6 A resolution.Each monomer (Mr 28K)is composed of an unusual arrangement of nine antiparallel alpha-helices organized as two structural domains.The dimer
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