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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSMBIO
- 检测范围:
0.15-10ng/ml
- 检测方法:
夹心法/竞争法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Matrix extracellular phosphoglycoprotein, MEPE
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078ng/ml
- 规格:
96T
Human Matrix extracellular phosphoglycoprotein, MEPE ELISA KIT
Product Name:Human Matrix extracellular phosphoglycoprotein, MEPE ELISA KIT
Packing:96T
Catalog No.:ELI-04077h
Gene Name:MEPE
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Matrix extracellular phosphoglycoprotein(MEPE
Alternative Name:MEPE,Matrix extracellular phosphoglycoprotein(MEPE
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Matrix extracellular phosphoglycoprotein, MEPE ELISA KIT allows for the in vitro quantitative determination of Matrix extracellular phosphoglycoprotein(MEPE concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Matrix extracellular phosphoglycoprotein, MEPE ELISA KIT has been pre-coated with an Matrix extracellular phosphoglycoprotein(MEPE antibody specific to Matrix extracellular phosphoglycoprotein(MEPE .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Matrix extracellular phosphoglycoprotein(MEPE and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Matrix extracellular phosphoglycoprotein(MEPE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Matrix extracellular phosphoglycoprotein(MEPE in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验. The application of this bovine corneal extracellular matrix (bECM) has since been greatly expanded (5 ,6 ). bECM has found broad approval, and has been used for mammary carcinoma (7 ), urological tumors (8 ), and different kinds of pituitary adenomas (9 ,10
Human embryonic stem cell (hESc) lines are likely the in vitro equivalent of the pluripotent epiblast. hESc express high levels of the extracellular matrix (ECM) laminin integrin receptor α6β1 and consequently can adhere robustly
even in vitro, bovine cornea1 endothelial basement membrane was explored by Gospodarowicz et al. for its role in regeneration and nonregeneration of cornea1 endothelium in different species This bovine cornea1 extracellular matrix (bECM) was found useful
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