- ￥2890
- LSM Bio
- 0.15-10ng/ml
- 0.078ng/ml
- 国产(香港）
- ELI-03275h
- 2026年04月29日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
20
- 供应商：
LSMBIO
- 检测范围：
0.15-10ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Catenin beta- 1, CTNNB1
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078ng/ml
- 规格：
96T
Human Catenin beta- 1, CTNNB1 ELISA KIT
Product Name:Human Catenin beta- 1, CTNNB1 ELISA KIT
Packing:96T
Catalog No.:ELI-03275h
Gene Name:CTNNB1
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:Catenin beta-1(CTNNB1
Alternative Name:CTNNB1,Catenin beta-1(CTNNB1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Catenin beta- 1, CTNNB1 ELISA KIT allows for the in vitro quantitative determination of Catenin beta-1(CTNNB1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Catenin beta- 1, CTNNB1 ELISA KIT has been pre-coated with an Catenin beta-1(CTNNB1 antibody specific to Catenin beta-1(CTNNB1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Catenin beta-1(CTNNB1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Catenin beta-1(CTNNB1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Catenin beta-1(CTNNB1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验/total beta-catenin处理后才代表beta-catenin的活性水平。 如检测核内水平， LZ需要做subcellular fractionation, 这个恐怕要买kit，自己配的话，一般很难过关，所以工作后者很能还更大，但是如果是我，我两种方法都用，因为想发好文章就要用不同的方法，无可辩驳的证据证明自己的结论，当然，差一些的文章，上述两种，选一即可。 lope 不好意思前几天休假忘了看了，谢谢楼上的赐教！ 果冻心-mm
v468.Chapter 8 采用 b-Catenin/TCF基本转录通讯结构对b-Catenin/TCF转录进行分析
., Morin, P.J., van Wichen, D., de Weger, R., Kinzler, K.W., Vogelstein, B., and Clevers, H. (1997). Constitutive transcriptional activation by a beta-catenin-Tcf complex in APC –/– colon carcinoma. Science 275 , 1784–1787. 5. Veeman, M.T., Slusarski
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9. GlutaMAX™-I Supplement 10. Basic FGF, Recombinant Human 11. β-Mercaptoethanol, 1000X 12. Penicillin-Streptomycin, Liquid 13. Attachment Factor 14. TrypLE™ Select Cell Dissociation Reagent or 0.05% Trypsin/EDTA
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