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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.15-10ng/ml
- 检测方法:
夹心法/竞争法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
T- cell surface glycoprotein CD4, CD4
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078ng/ml
- 规格:
96T
Human T- cell surface glycoprotein CD4, CD4 ELISA KIT
Product Name:Human T- cell surface glycoprotein CD4, CD4 ELISA KIT
Packing:96T
Catalog No.:ELI-03251h
Gene Name:CD4
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:T-cell surface glycoprotein CD4(CD4
Alternative Name:CD4,T-cell surface glycoprotein CD4(CD4
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human T- cell surface glycoprotein CD4, CD4 ELISA KIT allows for the in vitro quantitative determination of T-cell surface glycoprotein CD4(CD4 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human T- cell surface glycoprotein CD4, CD4 ELISA KIT has been pre-coated with an T-cell surface glycoprotein CD4(CD4 antibody specific to T-cell surface glycoprotein CD4(CD4 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for T-cell surface glycoprotein CD4(CD4 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain T-cell surface glycoprotein CD4(CD4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of T-cell surface glycoprotein CD4(CD4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验and H2-A and E in mice) for CD4+ T cells. However, a significant proportion of T cells recognize peptide antigens bound to nonclassical MHC class Ib molecules such as the human HLA-E (mouse analog Qa1) (4 ) and mouse H2-M3 (5 ). In addition, some T
度的功能细胞。二、人外周血中 CD4 T 细胞分选实例1.产品信息: MojoSort™ Human CD4 T Cell Isolation Kit(BioLegend, Cat#480009),阴性分选试剂盒2.检测仪器型号:BD LSRⅡ3.样本:正常人外周血4.分选前后检测相关产品:PE-CD4(Biolegend, Cat# 317410)5.实验过程(1)采血之后采用达优™ 人淋巴细胞分离液分离人外周血单个核细胞。分离之后能见到明显的白膜层。(达优,Cat#DKW-KLSH-0100
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