- ￥2890
- LSM Bio
- 0.15-10ng/ml
- 0.078ng/ml
- 国产
- ELI-03185h
- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.15-10ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Cytochrome P450 2E1, CYP2E1
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078ng/ml
- 规格：
96T
Human Cytochrome P450 2E1, Cyp2e1 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Cytochrome P450 2E1,Cytochrome P450RLM6,Cytochrome P450-J,4-nitrophenol 2-hydroxylase,CYPIIE1, Cyp2e1,cytochrome P450 2E1; CPE1; CYP2E; P450-J; P450C2E; 4-nitrophenol 2-hydroxylase; CYPIIE1; cytochrome P450 subfamily IIE ethanol-inducible polypeptide 1; cytochrome P450-J; flavoprotein-linked monooxygenase; microsomal monooxygenase; xenobiotic monooxygenase; cytochrome P450 family 2 subfamily E polypeptide 1
Search name
Human Cytochrome P450 2E1 ELISA KIT ,Human Cytochrome P450RLM6 ELISA KIT ,Human Cytochrome P450-J ELISA KIT ,Human 4-nitrophenol 2-hydroxylase ELISA KIT ,Human CYPIIE1 ELISA KIT ,Human Cyp2e1 ELISA KIT ,Human cytochrome P450 2E1 ELISA KIT ,Human CPE1 ELISA KIT ,Human CYP2E ELISA KIT ,Human P450-J ELISA KIT ,Human P450C2E ELISA KIT ,Human 4-nitrophenol 2-hydroxylase ELISA KIT ,Human CYPIIE1 ELISA KIT ,Human cytochrome P450 subfamily IIE ethanol-inducible polypeptide 1 ELISA KIT ,Human cytochrome P450-J ELISA KIT ,Human flavoprotein-linked monooxygenase ELISA KIT ,Human microsomal monooxygenase ELISA KIT ,Human xenobiotic monooxygenase ELISA KIT ,Human cytochrome P450 family 2 subfamily E polypeptide 1 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Human Cytochrome P450 2E1 concentrations in serum, Plasma,, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome P450 2E1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Cytochrome P450 2E1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Cytochrome P450 2E1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Cytochrome P450 2E1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Evaluation of Cytochrome P450 Activities in Human Hepatocytes In Vitro
Major hepatic cytochrome P450 activities (CYP1A2, CYP2A6, CYP2B6, CYP2C9, CYP2C19, CYP2D6, CYP2E1, and CYP3A4) can be simultaneously examined in human hepatocytes by incubation with a cocktail of multiple specific probes. Cocktail strategy
Genotyping for Cytochrome P450 Polymorphisms
Protocols for the extraction of DNA from human blood and for genotyping for a number of common cytochrome P450 polymorphisms using either polymerase chain reaction (PCR)-restriction fragment length polymorphism or PCR-single-strand
Catalytic Assays for Human Cytochrome P450
Cytochrome P450 (P450) enzymes catalyze the blotransformatlon of a broad range of structurally diverse foreign chemicals and endogenous substances. Many P450 enzymes have been isolated and identified in liver and other tlssues, including
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