Human Glypican-3,GPC3 ELISA KI

Human Glypican-3, Gpc3 ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Gpc3,DGSX; GTR2-2; MXR7; OCI-5; SDYS; SGB; SGBS; SGBS1; glypican proteoglycan 3; glypican-3; heparan sulphate proteoglycan; intestinal protein OCI-5; secreted glypican-3; glypican 3

Search name

Human Gpc3 ELISA KIT ,Human DGSX ELISA KIT ,Human GTR2-2 ELISA KIT ,Human MXR7 ELISA KIT ,Human OCI-5 ELISA KIT ,Human SDYS ELISA KIT ,Human SGB ELISA KIT ,Human SGBS ELISA KIT ,Human SGBS1 ELISA KIT ,Human glypican proteoglycan 3 ELISA KIT ,Human glypican-3 ELISA KIT ,Human heparan sulphate proteoglycan ELISA KIT ,Human intestinal protein OCI-5 ELISA KIT ,Human secreted glypican-3 ELISA KIT ,Human glypican 3 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of human Glypican-3 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glypican-3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Glypican-3 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Glypican-3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Glypican-3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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