- ￥2890
- LSM Bio
- 0.15-10ng/ml
- 0.078ng/ml
- 国产(香港）
- ELI-02668h
- 2026年05月21日
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- 文献和实验
- 技术资料
- 库存：
20
- 供应商：
LSMBIO
- 检测范围：
0.15-10ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Carbonic anhydrase 2, CA2
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078ng/ml
- 规格：
96T
Human carbonic anhydrase 2, CA2 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
Carbonic anhydrase 2,; Carbonate dehydratase II,Carbonic anhydrase II(CA-II),; Car2; AI131712; CAII; Ca2; Car-2; Ltw-5; Lvtw-5; CA II;
CA-II;
Search name
Human carbonic anhydrase 2 ELISA Kit, Human CA-2 ELISA Kit, Human Carbonate dehydratase II ELISA Kit, Human CA-II
ELISA Kit, Human Car2 ELISA Kit, Human AI131712 ELISA Kit, Human Car-2 ELISA Kit, Human Ltw-5 ELISA Kit, Human
CA2 ELISA Kit, Human Lvtw-5 ELISA Kit, Human CA II ELISA Kit,
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Human Carbonic anhydrase 2 concentrations in serum, Plasma,
tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Carbonic anhydrase 2. Standards or samples are
then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Carbonic
anhydrase 2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB
substrate solution is added to each well. Only those wells that contain Carbonic anhydrase 2, biotin-conjugated antibody and
enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid
solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Carbonic
anhydrase 2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验samples, MPC-15 for 1–15 ml samples and MPC-50 for 15–50 ml samples. Mixer allowing both tilting and rotation. Buffer 1: PBS (without Ca2 and Mg2 ) w/0.1% BSA and 2 mM EDTA, pH 7.4. Buffer 2: PBS pH 7.4 (without Ca2 and Mg2 ).
调至 1μg/μl。 4. ELISA 检测：按试剂盒说明书完成板平衡、加样孵育、洗板、酶标抗体孵育、显色终止与读数，结合标准曲线得出 IFN-γ 浓度。 四、操作要点速记 1.组织样本新鲜处理，全程低温操作，裂解液现配现用； 2. 蛋白定量时标准品与样本同步孵育，稀释采用倍比稀释减少误差； 3. ELISA 实验需每次重绘标准曲线，洗板充分、严格把控显色与终止时间。 免疫检测试剂盒： 货号-规格 名称 abs510001-96T Human IL-2 ELISA Kit (Faster
PriCells: Introduction of Isolation and Culture of Human Alveolar Epithelial Cells
PriCells: Introduction of Isolation and Culture of Human Alveolar Epithelial Cells 1.1. Background The human lung consists of more than 40 different cell types. The morphology and function of constituent cells of the proximal, conducting airway
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