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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSMBIO
- 检测范围:
0.15-10ng/ml
- 检测方法:
夹心法/竞争法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Hepatic triacylglycerol lipase, LIPC
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078ng/ml
- 规格:
96T
Human Hepatic triacylglycerol lipase, LIPC ELISA KIT
Product Name:Human Hepatic triacylglycerol lipase, LIPC ELISA KIT
Packing:96T
Catalog No.:ELI-02629h
Gene Name:LIPC
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Hepatic triacylglycerol lipase(LIPC
Alternative Name:LIPC,Hepatic triacylglycerol lipase(LIPC
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Hepatic triacylglycerol lipase, LIPC ELISA KIT allows for the in vitro quantitative determination of Hepatic triacylglycerol lipase(LIPC concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Hepatic triacylglycerol lipase, LIPC ELISA KIT has been pre-coated with an Hepatic triacylglycerol lipase(LIPC antibody specific to Hepatic triacylglycerol lipase(LIPC .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Hepatic triacylglycerol lipase(LIPC and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Hepatic triacylglycerol lipase(LIPC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Hepatic triacylglycerol lipase(LIPC in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验High-Level Expression and Purification of Human Hepatic Lipase from Mammalian Cells
Through its ability to catalyze the hydrolysis of triglycerides and phospholipids, hepatic lipase (HL) has been shown to influence the metabolism of a broad range of circulating lipoproteins (1 ). HL is synthesized and secreted
Purification of Rat Hepatic Lipase Essentially Free of Apolipoprotein E and Apolipoprotein B
subfractions. In isolated, perfused rat livers inhibition of hepatic lipase with antibodies or removal of HL by heparin perfusion reduces the uptake of chylomicron remnants (13 ). Finally, recent studies with cultured hepatoma cells over-expressing human HL
Hormone-Sensitive Lipase and Neutral Cholesteryl Ester Lipase
Hormone-sensitive lipase (HSL) catalyzes the rate-limiting step in the hydrolysis of stored triacylglycerols in adipocytes, i.e., the hydrolysis of triacylglycerol to diacylglycerol (for a recent review on HSL, see ref
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