Human Glucose-6-phosphate isom

Human Glucose- 6- phosphate isomerase, GPI ELISA KIT

Product Name:Human Glucose- 6- phosphate isomerase, GPI ELISA KIT
Packing:96T

Catalog No.:ELI-02516h

Gene Name:GPI

Detect Range:78-5000 pg/mL

Sensitivity:32pg/mL

Target Protein Name:Glucose-6-phosphate isomerase(GPI

Alternative Name:GPI,Glucose-6-phosphate isomerase(GPI
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human Glucose- 6- phosphate isomerase, GPI ELISA KIT allows for the in vitro quantitative determination of Glucose-6-phosphate isomerase(GPI concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human Glucose- 6- phosphate isomerase, GPI ELISA KIT has been pre-coated with an Glucose-6-phosphate isomerase(GPI antibody specific to Glucose-6-phosphate isomerase(GPI .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Glucose-6-phosphate isomerase(GPI and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Glucose-6-phosphate isomerase(GPI, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Glucose-6-phosphate isomerase(GPI in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!