- ￥2890
- LSM Bio
- 0.15-10ng/ml
- 0.078ng/ml
- 国产
- ELI-02487h
- 2025年07月04日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.15-10ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Mu- type opioid receptor, OPRM1
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078ng/ml
- 规格：
96T
Human Mu- type opioid receptor, OPRM1 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
OPRM1,mu opioid receptor,LMOR; hMOP ,M-OR-1; MOP; MOR; MOR1; OPRM; micro opioid receptor isoform hMOR-1A2; mu opiate receptor; mu opioid receptor hMOR-1a; mu-type opioid receptor; opioid receptor mu 1 Mu-type opioid receptor,M-OR-1,MOR-1
Search name
Human OPRM1 ELISA KIT ,Human mu opioid receptor ELISA KIT ,Human LMOR ELISA KIT ,Human hMOP ELISA KIT ,Human M-OR-1 ELISA KIT ,Human MOP ELISA KIT ,Human MOR ELISA KIT ,Human MOR1 ELISA KIT ,Human OPRM ELISA KIT ,Human micro opioid receptor isoform hMOR-1A2 ELISA KIT ,Human mu opiate receptor ELISA KIT ,Human mu opioid receptor hMOR-1a ELISA KIT ,Human mu-type opioid receptor ELISA KIT ,Human opioid receptor mu 1 Mu-type opioid receptor ELISA KIT ,Human M-OR-1 ELISA KIT ,Human MOR-1 ELISA KIT
Intended use
Human OPRM1 ELISA KIT allows for the in vitro quantitative determination of human mu-type opioid receptor,M-OR-1 concentrations in serum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in Human OPRM1 ELISA KIT has been pre-coated with an antibody specific to M-OR-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for M-OR-1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain M-OR-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of M-OR-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Mu-opioid receptor (MOR) belongs to a family of heptahelical G-protein-coupled receptors (GPCRs). Studies in humans and rodents demonstrated that the OPRM1 gene coding for MOR undergoes extensive alternative splicing afforded
In Vitro Opioid Receptor Assays
analgesics have been used for centuries, identification of opioid receptors and the ability of an opioid receptor antagonist to block natural pain processes prompted a search for endogenous opioid peptides. In vitro models were needed to characterize opioid
This protocol is based on enrichment from 1 x 107 leucocytes (MNC). It is scalable from 1 x 107 –2 x 109 cells. Keep the cells and buffers cold (2–8°C) during the whole process. 1) Transfer 100 μl (1 x 107 ) leucocytes in Buffer
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