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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.15-10ng/ml
- 检测方法:
夹心法/竞争法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
BH3- interacting domain death agonist, BID
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078ng/ml
- 规格:
96T
Human BH3- interacting domain death agonist, BID ELISA KIT
Product Name:Human BH3- interacting domain death agonist, BID ELISA KIT
Packing:96T
Catalog No.:ELI-02236h
Gene Name:BID
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:BH3-interacting domain death agonist(BID
Alternative Name:BID,BH3-interacting domain death agonist(BID
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human BH3- interacting domain death agonist, BID ELISA KIT allows for the in vitro quantitative determination of BH3-interacting domain death agonist(BID concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human BH3- interacting domain death agonist, BID ELISA KIT has been pre-coated with an BH3-interacting domain death agonist(BID antibody specific to BH3-interacting domain death agonist(BID .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for BH3-interacting domain death agonist(BID and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain BH3-interacting domain death agonist(BID, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of BH3-interacting domain death agonist(BID in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验相关专题 细胞凋亡专题 线粒体 凋亡途径是细胞凋亡的主要途径之一,是目前研究凋亡的热点.各种凋亡刺激信号通过BH3(Bcl-2 homology domain 3)-only蛋白引起Bax(Bcl-2-asslciated protein X)蛋白移位到线粒体外膜并多聚化,形成膜通道,刺激线粒体释放细胞色素C(Cyt C) 和Smac(second mitochondrial-derived activator
. In many cases, information from the environment is relayed to a central 'apoptosis rheostat' at the mitochondrion by stimulus-specific induction, modification or movement of pro-apoptotic 'BH3 domain-only' members of the Bcl-2 family (including
surface protein containing the CS1 domain contributes to binding of lymphoblasts. J. Neurosci. Res. 50:539-548.[3] Chen Y., and Swanson, R. A. (2003) Astrocytes and brain injury. J. Cereb. Blood Flow Metab. 23:137-149.Human MicrogliaMicroglia
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