- ￥2890
- LSM Bio
- 0.15-10ng/ml
- 0.078ng/ml
- 国产
- ELI-02115h
- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.15-10ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Pyruvate kinase isozymes M1/M2, PKM2
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078ng/ml
- 规格：
96T
Human Pyruvate kinase isozymes M1/M2, PKM2 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
M2- PK,PKM,CTHBP,HEL-S-30,OIP3,PK3,PKM2,TCB,THBP1,OIP-3,OPA-interacting protein 3,PK muscle type,cytosolic thyroid hormone-binding protein,epididymis secretory protein Li 30,p58,pyruvate kinase 2/3,pyruvate kinase PKM,pyruvate kinase isozymes M1/M2,pyruvate kinase muscle isozyme,thyroid hormone-binding protein 1,thyroid hormone-binding protein cytosolic,tumor M2-PK,pyruvate kinase muscle,Tumor M2-PK,Thyroid hormone-binding protein 1,THBP1,Pyruvate kinase muscle isozyme,Pyruvate kinase 2/3,p58,Cytosolic thyroid hormone-binding protein,CTHBP,Opa-interacting protein 3
Search name
Human PKM2 ELISA KIT ,Human M2- PK ELISA KIT ,Human M2PK ELISA KIT,Human PKM ELISA KIT ,Human CTHBP ELISA KIT ,Human HEL-S-30 ELISA KIT ,Human OIP3 ELISA KIT ,Human PK3 ELISA KIT ,Human TCB ELISA KIT ,Human THBP1 ELISA KIT ,Human OIP-3 ELISA KIT ,Human OPA-interacting protein 3 ELISA KIT ,Human PK muscle type ELISA KIT ,Human cytosolic thyroid hormone-binding protein ELISA KIT ,Human epididymis secretory protein Li 30 ELISA KIT ,Human p58 ELISA KIT ,Human pyruvate kinase 2/3 ELISA KIT ,Human pyruvate kinase PKM ELISA KIT ,Human pyruvate kinase isozymes M1/M2 ELISA KIT ,Human pyruvate kinase muscle isozyme ELISA KIT ,Human thyroid hormone-binding protein 1 ELISA KIT ,Human thyroid hormone-binding protein cytosolic ELISA KIT ,Human tumor M2-PK ELISA KIT ,Human pyruvate kinase muscle ELISA KIT ,Human Tumor M2-PK ELISA KIT ,Human Thyroid hormone-binding protein 1 ELISA KIT ,Human THBP1 ELISA KIT ,Human Pyruvate kinase muscle isozyme ELISA KIT ,Human Pyruvate kinase 2/3 ELISA KIT ,Human p58 ELISA KIT ,Human Cytosolic thyroid hormone-binding protein ELISA KIT ,Human CTHBP ELISA KIT ,Human Opa-interacting protein 3 ELISA KIT
Intended use
Human PKM2 ELISA KIT allows for the in vitro quantitative determination of human Pyruvate kinase isozymes M1/M2 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in Human PKM2 ELISA KIT has been pre-coated with an antibody specific to Pyruvate kinase isozymes M1/M2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Pyruvate kinase isozymes M1/M2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Pyruvate kinase isozymes M1/M2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Pyruvate kinase isozymes M1/M2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human PKM2 ELISA KIT range 1.56-100ng/ml
Human PKM2 ELISA KIT Human-Pyruvate-kinase-isozymes-M1-M2-PKM2-ELISA-KIT
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文献和实验Using Phospho‐Motif Antibodies to Determine Kinase Substrates
Manning, G., Whyte, D.B., Martinez, R., Hunter, T., and Sudarsanam, S. 2002b. The protein kinase complement of the human genome. Science 298:1912‐1934.
Assay (Invitrogen) as suggested by Illumina. Illumina Bead Array MicroRNA Detection Starting with 500 ng/sample of total RNA, mature microRNAs were amplified with the Illumina human v1 MicroRNA expression profiling kit containing primers for 743
[资源] 所有的看家基因(housekeeping genes)列表+引物设计服务
pyruvate kinase, muscle (PKM2), mRNA 2474 NM_002648 Homo sapiens pim-1 oncogene (PIM1), mRNA 1052 NM_002635 Homo sapiens solute carrier family 25 (mitochondrial carrier; phosphate carrier), member 3 (SLC25A3), nuclear gene encoding mitochondrial
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