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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.15-10ng/ml
- 检测方法:
夹心法/竞争法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Retinoic acid/ Tretinoin,RA
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078ng/ml
- 规格:
96T
Human Retinoic acid/ Tretinoin,RA ELISA Kit
Product Name:Human Retinoic acid/ Tretinoin,RA ELISA Kit
Packing:96T
Catalog No.:ELA-E2553h
Gene Name:RA
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Retinoic acid(Tretinoin,RA
Alternative Name:RA,Retinoic acid(Tretinoin,RA
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Retinoic acid/ Tretinoin,RA ELISA Kit allows for the in vitro quantitative determination of Retinoic acid(Tretinoin,RA concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Retinoic acid/ Tretinoin,RA ELISA Kit has been pre-coated with an Retinoic acid(Tretinoin,RA antibody specific to Retinoic acid(Tretinoin,RA .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Retinoic acid(Tretinoin,RA and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Retinoic acid(Tretinoin,RA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Retinoic acid(Tretinoin,RA in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Directed Differentiation of Human Embryonic Stem Cells to Epidermal Progenitors
subpopulations are required. Here we describe a robust strategy for generating cytokeratin 14+ (K14+)/p63+ keratinocyte progenitors from hES cells through stage-specific application of retinoic acid (RA) and bone morphogenetic protein-4 (BMP4). Induction
Generation of Functional Insulin-Producing Cells from Human Embryonic Stem Cells In Vitro
system, which could induce human ES cells to differentiate into functional insulin-producing cells. Activin A was first used in the initial stage to induce definitive endoderm lineage differentiation from human ES cells. And all-trans Retinoic Acid (RA) was then utilized
预期应用本试剂盒运用双抗体夹心 ELISA 法定量测定人组织匀浆、细胞裂解液和其他生物液体中 IL8Ra 含量。试剂盒内容试剂名称数量试剂名称数量96孔板(预包被)196孔板覆膜2标准品2标准品稀释液1×20mL检测溶液A1×120μL检测稀释液A1×12mL检测溶液B1×120μL检测稀释液B1×12mLTMB底物1×9mL终止液1×6mL浓洗涤液(30×)1×20mL使用说明书1需自备的设备及试剂1.450±10nm 滤光片的酶标仪 (建议仪器使用前提前预热)2.单道或多道微量加液器及吸头
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