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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.15-10ng/ml
- 检测方法:
夹心法/竞争法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
IL36G
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078ng/ml
- 规格:
96T
Human IL36G ELISA Kit
Product Name:Human IL36G ELISA Kit
Packing:96T
Catalog No.:ELA-E2205h
Gene Name:IL36G
Detect Range:31.2-2000 pg/mL
Sensitivity:10pg/mL
Target Protein Name:Human Interleukin-36 gamma
Alternative Name:Interleukin-36 gamma, IL-1-related protein 2, IL-1RP2, Interleukin-1 epsilon, IL-1 epsilon, Interleukin-1 family member 9, IL-1F9, Interleukin-1 homolog 1, IL-1H1, IL36G, IL1E, IL1F9, IL1H1,
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human IL36G ELISA Kit allows for the in vitro quantitative determination of Human Interleukin-36 gamma concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human IL36G ELISA Kit has been pre-coated with an IL36G antibody specific to Human Interleukin-36 gamma .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Interleukin-36 gamma and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Interleukin-36 gamma, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human Interleukin-36 gamma in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验volume of 35 ml at room temperature. 2) Add the diluted buffy coat on top of 15 ml of Lymphoprep. 3) Centrifuge at 160 x g for 20 minutes at 20°C. Allow to decellerate without brakes. 4) Remove 20 ml of supernatant
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