Human APOM ELISA Kit

Human APOM ELISA Kit

Product Name:Human APOM ELISA Kit
Packing:96T

Catalog No.:ELA-E2167h

Gene Name:APOM

Detect Range:2.5-160 ng/mL

Sensitivity:1.1 ng/mL

Target Protein Name:Human Apolipoprotein M

Alternative Name:Apolipoprotein M, Apo-M, ApoM, Protein G3a, APOM, G3A, NG20, HSPC336
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human APOM ELISA Kit allows for the in vitro quantitative determination of Human Apolipoprotein M concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human APOM ELISA Kit has been pre-coated with an APOM antibody specific to Human Apolipoprotein M .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Apolipoprotein M and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Apolipoprotein M, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human Apolipoprotein M in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!