- ￥2890
- LSM Bio
- 0.15-10ng/ml
- 0.078ng/ml
- 国产
- ELA-E2160h
- 2025年07月10日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.15-10ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Sphingosine
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078ng/ml
- 规格：
96T
Human Sphingosine, SPH ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
Sphingosine,; 2-amino-4-octadecene-1,3-diol
Search name
Human Sphingosine ELISA Kit, Human 2-amino-4-octadecene-1,3-diol ELISA Kit, Human Sph ELISA Kit, Sphingosine ELISA Kit,
2-amino-4-octadecene-1,3-diol ELISA Kit, Sph ELISA Kit
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Sphingosine concentrations in serum, plasma, tissue
homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to Sphingosine, During the reaction, Sphingosine in the sample or standard competes with a fixed
amount of biotin-labeled Sphingosine for sites on a pre-coated Monoclonal antibody specific to Sphingosine. Excess conjugate and
unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each
microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the
addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The
concentration of Sphingosine in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PDF manual download
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文献和实验Sphingosine-1-phosphate (S1P) and the enzyme primarily responsible for its production, sphingosine kinase-1 (SphK-1), are thought to be dysregulated in multiple human diseases including cancer, multiple sclerosis (MS), diabetes, neurological
Warnings And Limitations This product is for research use only. Not intended for any animal or human therapeutic or diagnostic use unless otherwise stated. Follow appropriate laboratory guidelines. This product contains 0.02% sodium azide
RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
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