Human IL36B ELISA Kit
Product Name:Human IL36B ELISA Kit
Packing:96T
Catalog No.:ELA-E2013h
Gene Name:IL36B
Detect Range:78-5000 pg/mL
Sensitivity:31pg/mL
Target Protein Name:Human Interleukin-36 beta
Alternative Name:Interleukin-36 beta, FIL1 eta, Interleukin-1 eta, IL-1 eta, Interleukin-1 family member 8, IL-1F8, Interleukin-1 homolog 2, IL-1H2, IL36B, IL1F8, IL1H2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human IL36B ELISA Kit allows for the in vitro quantitative determination of Human Interleukin-36 beta concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human IL36B ELISA Kit has been pre-coated with an IL36B antibody specific to Human Interleukin-36 beta .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Interleukin-36 beta and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Interleukin-36 beta, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human Interleukin-36 beta in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!