- ￥2890
- LSM Bio
- 0.15-10ng/ml
- 0.078mg/ml
- 国产
- ELA-E1807h
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.15-10ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Plasma fibronectin
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078mg/ml
- 规格：
96T
Human Plasma fibronectin ELISA Kit
Product Name:Human Plasma fibronectin ELISA Kit
Packing:96T
Catalog No.:ELA-E1807h
Gene Name:pFN
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Plasma fibronectin
Alternative Name:pFN,Plasma fibronectin
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Plasma fibronectin ELISA Kit allows for the in vitro quantitative determination of Plasma fibronectin concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Plasma fibronectin ELISA Kit has been pre-coated with an Plasma fibronectin antibody specific to Plasma fibronectin .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Plasma fibronectin and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Plasma fibronectin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Plasma fibronectin in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Purification of Fibronectin from Human Plasma
for each cell and tissue. Although blood cells, such as lymphocytes, myelocytes, and erythrocytes, lack fibronectin, this gly-coprotein is abundant in the plasma (about 30 mg/100 mL). Actually, this body fluid represents the main source for fibronectin
to eliminate platelets. 5) Centrifuge at 350 x g for 20 minutes at 20°C. Allow to decellerate without brakes. 6) Recover MNC from the plasma/Lymphoprep interface and transfer the cells to a 50 ml tube. 7) Wash MNC
RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
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