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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSMBIO
- 检测范围:
0.15-10ng/ml
- 检测方法:
夹心法/竞争法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Total protein S
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078mg/ml
- 规格:
96T
Human Total protein S ELISA Kit
Product Name:Human Total protein S ELISA Kit
Packing:96T
Catalog No.:ELA-E1801h
Gene Name:TPS
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Total protein S
Alternative Name:TPS,Total protein S
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Total protein S ELISA Kit allows for the in vitro quantitative determination of Total protein S concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Total protein S ELISA Kit has been pre-coated with an Total protein S antibody specific to Total protein S .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Total protein S and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Total protein S, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Total protein S in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Reproducible purification of DNA and RNA in 96-well format. Genomic DNA and total RNA were purified from mouse tissues using the AllPrep DNA/RNA 96 Kit. A Purified DNA samples were analyzed on a 0.8% agarose gel. B
Isolation of Total RNA from Difficult Tissues
techniques to help overcome problems associated with isolating total RNA from difficult tissues. The tips provided are based on RNA isolation by a guanidinium thiocyanate/acid phenol:chloroform extraction method (e.g. Ambion"s ToTALLY RNA™ Kit). Note
the beadbound cells with a magnet. Discard the bead-bound cells and use the remaining untouched, enriched cell population for further flow sorting into the DC subpopulation(s) of interest 实验试剂 Magnet: (Dynal® MPC™), MPC-L for 1–5 ml
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