- ￥2890
- LSM Bio
- 0.15-10ng/ml
- 0.078mg/ml
- 国产(香港）
- ELA-E1977h
- 2026年05月21日
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- 文献和实验
- 技术资料
- 库存：
20
- 供应商：
LSMBIO
- 检测范围：
0.15-10ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Carboxymethyl lysine
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078mg/ml
- 规格：
96T
Human Carboxymethyl lysine, CML ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
N(6)-Carboxymethyllysine,; N(epsilon)-(carboxymethyl)lysine, CML , Carboxymethyl lysine
Search name
Human Carboxymethyl lysine ELISA Kit, Human CML ELISA Kit, Human N(6)-Carboxymethyllysine ELISA Kit, Human
N(epsilon)-(carboxymethyl)lysine ELISA Kit, Carboxymethyl lysine ELISA Kit, CML ELISA Kit, N(6)-Carboxymethyllysine ELISA
Kit, N(epsilon)-(carboxymethyl)lysine ELISA Kit,
Intended use
This immunoassay kit allows for the in vitro quantitative determination of
N(6)-Carboxymethyllysine concentrations in serum, plasma,
tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to N(6)-Carboxymethyllysine, During the reaction, N(6)-Carboxymethyllysine in the sample or
standard competes with a fixed amount of biotin-labeled N(6)-Carboxymethyllysine for sites on a pre-coated Monoclonal antibody specific
to N(6)-Carboxymethyllysine. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to
Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The
enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of N(6)-Carboxymethyllysine in the samples is then
determined by comparing the O.D. of the samples to the standard curve.
PDF manual download
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文献和实验An amide-type adduct, hexanoyl-lysine (HEL) is generated from the reaction between n-6 fatty acid (FA)-derived lipid peroxide and lysine. Immunochemical and chemical methods can be used to detect the formation of HEL. For example, an ELISA
调至 1μg/μl。 4. ELISA 检测：按试剂盒说明书完成板平衡、加样孵育、洗板、酶标抗体孵育、显色终止与读数，结合标准曲线得出 IFN-γ 浓度。 四、操作要点速记 1.组织样本新鲜处理，全程低温操作，裂解液现配现用； 2. 蛋白定量时标准品与样本同步孵育，稀释采用倍比稀释减少误差； 3. ELISA 实验需每次重绘标准曲线，洗板充分、严格把控显色与终止时间。 免疫检测试剂盒： 货号-规格 名称 abs510001-96T Human IL-2 ELISA Kit (Faster
操作条件、人员一致 边缘效应 蒸发/酶标板叠放 孵育加封板膜或盖板；避免叠放 孵育温度不均 校准温育箱；避开通风口、窗边等环境波动区 洗板不彻底 确保洗板仪正常；不减少洗液量与次数 注意！！！ ELISA试剂盒11月活动即将来袭~敬请期待~ 部分热卖产品推荐 产品货号 产品名称 检测范围 灵敏度 97109ES Human IL18 ELISA Kit 125
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