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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.31-20ng/ml
- 检测方法:
夹心法/竞争法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
allopregnanolone/ 3a,5a- tetrahydroprogesterone, AP/ 3a,5a
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.1ng/ml
- 规格:
96T
Human allopregnanolone/ 3a,5a- tetrahydroprogesterone, AP/ 3a,5a- THP ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
Allopregnanolone,3a,5a-tetrahydroprogesterone,3a-hydroxy-5a-pregnan-20-one,
Search name
Human Allopregnanolone ELISA Kit, Allopregnanolone ELISA Kit, Human 3a,5a-tetrahydroprogesterone ELISA Kit,
3a,5a-tetrahydroprogesterone ELISA Kit,
Intended use
This immunoassay kit allows for the in vitro quantitative determination of allopregnanolone,Allopregnanolone concentrations in
serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to Allopregnanolone, During the reaction, Allopregnanolone in the sample or standard competes with
a fixed amount of biotin-labeled Allopregnanolone for sites on a pre-coated Monoclonal antibody specific to Allopregnanolone. Excess
conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is
added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is
terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450
nm ± 2 nm. The concentration of Allopregnanolone in the samples is then determined by comparing the O.D. of the samples to the
standard curve.
PDF manual download
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