- ￥2890
- LSM Bio
- 0.15-10ng/ml
- 0.078mg/ml
- 国产
- ELA-E1561h
- 2025年07月10日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.15-10ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
leukocyte antigen A
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078mg/ml
- 规格：
96T
Human leukocyte antigen A ELISA Kit
Product Name:Human leukocyte antigen A ELISA Kit
Packing:96T
Catalog No.:ELA-E1561h
Gene Name:HLA-A
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:leukocyte antigen A
Alternative Name:HLA-A,leukocyte antigen A
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human leukocyte antigen A ELISA Kit allows for the in vitro quantitative determination of leukocyte antigen A concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human leukocyte antigen A ELISA Kit has been pre-coated with an leukocyte antigen A antibody specific to leukocyte antigen A .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for leukocyte antigen A and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain leukocyte antigen A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of leukocyte antigen A in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Human Leukocyte Antigen (HLA) Typing by DNA Sequencing
DNA sequencing is a powerful technique for identifying allelic variation within the human leukocyte antigen (HLA) genes. Sequencing is usually focused on the most polymorphic exons of the class I (HLA-A, -B, -C) and class II (HLA-DR, -DQ
人白细胞抗原(human leukocyte antigen，HLA)
gene)。人的定位MHC在第6号染色体上，小鼠的则定位在第17号染色体上。 组织相容性抗原按其免疫性能可分为三类：第一类是移植抗原(transplanta—tion antigen)，负责排斥外来的组织，存在于杀伤性T淋巴细胞(killer T leukocyte)中，为细胞介导的免疫反应所必需，小鼠的I类基因编码H2-K和H2-D／L蛋白质，人的I类基因编码移植抗原HLA-A，B，C；第二类是参与免疫反应过程中细胞之间的信息传递，人的Ⅱ类区称为HLA-D，又可分为4个亚区，排列顺序为DR，DQ
Pyrosequencing-Based Strategies for Improved Allele Typing of Human Leukocyte Antigen Loci
Successful transplantation of tissue during solid organ and bone marrow transplantation relies on accurate determination of the human leukocyte antigen (HLA ) phenotype of the potential donor(s) and recipient. Matching donor with recipient
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