Human Lipoxin A4 ELISA Kit

Human Lipoxin A4, LXA4 ELISA Kit
96 Tests
Operating instructions

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Lipoxin A4,;LXA4

Search name
Human LXA4 ELISA Kit, Human Lipoxin A4 ELISA Kit, Lipoxin A4 ELISA Kit,LXA4 ELISA Kit

Intended use
 
This immunoassay kit allows for the in vitro quantitative determination of
 
lipoxin a4,Lipoxin A4 concentrations in serum, plasma, tissue
 
homogenates, cell culture supernates, and other biological fluids.

Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to Lipoxin A4, During the reaction, Lipoxin A4 in the sample or standard competes with a fixed
amount of biotin-labeled Lipoxin A4 for sites on a pre-coated Monoclonal antibody specific to Lipoxin A4. Excess conjugate and unbound
sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate
well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of
a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration
of Lipoxin A4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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