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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.31-20ng/ml
- 检测方法:
夹心法/竞争法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
B- Cell Translocation Gene 2
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.1ng/ml
- 规格:
96T
Human B- Cell Translocation Gene 2 ELISA Kit
Product Name:Human B- Cell Translocation Gene 2 ELISA Kit
Packing:96T
Catalog No.:ELA-E14159h
Gene Name:BTG2
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:B-Cell Translocation Gene 2
Alternative Name:BTG2,B-Cell Translocation Gene 2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human B- Cell Translocation Gene 2 ELISA Kit allows for the in vitro quantitative determination of B-Cell Translocation Gene 2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human B- Cell Translocation Gene 2 ELISA Kit has been pre-coated with an B-Cell Translocation Gene 2 antibody specific to B-Cell Translocation Gene 2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for B-Cell Translocation Gene 2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain B-Cell Translocation Gene 2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of B-Cell Translocation Gene 2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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to the study of the antibody repertoire formed in vivo during, for example, autoimmune diseases. We describe here, the use of the in-cell PCR together with Cre-recombination applied to human B cells to obtain in situ pairing of the variable (V
Expression Cloning of Human B Cell Immunoglobulins
to obtain information on the clonal evolution of B cell lymphomas by single cell Ig gene sequencing and on the antibody reactivity of human lymphoma B cells.
Highly Proficient Gene Targeting by Homologous Recombination in the Human Pre-B Cell Line Nalm-6
for analyzing the functions of human genes in greater detail. Although the frequency of gene targeting is typically very low in human cultured cells, we have recently shown that a human precursor B cell line, Nalm-6, exceptionally allows for high-efficiency gene
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