- ￥2890
- LSM Bio
- 0.15-10ng/ml
- 0.078mg/ml
- 国产
- ELA-E13594h
- 2025年12月09日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.15-10ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Isocitrate Dehydrogenase 1, Soluble
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078mg/ml
- 规格：
96T
Human Isocitrate Dehydrogenase 1, Soluble ELISA Kit
Product Name:Human Isocitrate Dehydrogenase 1, Soluble ELISA Kit
Packing:96T
Catalog No.:ELA-E13594h
Gene Name:IDH1
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Isocitrate Dehydrogenase 1(Soluble
Alternative Name:IDH1,Isocitrate Dehydrogenase 1(Soluble
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Isocitrate Dehydrogenase 1, Soluble ELISA Kit allows for the in vitro quantitative determination of Isocitrate Dehydrogenase 1(Soluble concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Isocitrate Dehydrogenase 1, Soluble ELISA Kit has been pre-coated with an Isocitrate Dehydrogenase 1(Soluble antibody specific to Isocitrate Dehydrogenase 1(Soluble .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Isocitrate Dehydrogenase 1(Soluble and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Isocitrate Dehydrogenase 1(Soluble, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Isocitrate Dehydrogenase 1(Soluble in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
). Several substrates yield colored or fluorescent soluble products which are useful when quantifying ß-gal activity (McCaman and Robins, 1959, Miller 1972) or visualizing transduced cells live in vivo (Krasnow et al. 1991, Nirenberg and Cepko, 1993, Lin et al. 1994
就可进行一次检测。3、更高的灵敏度。分析灵敏度高达2.8pg/mL。4、更高的灵敏度、更宽的检测范围和更好的重复性。CBA技术使蛋白定量的分析灵敏度高达pg/ml级别,检测范围达0-5000pg/ml，所有分析只需一组标准曲线。根据抗体的荧光强度对目的蛋白定量，排除了ELISA反应中酶联放大产生的假阳性。5、检测对象的灵活组合—BD CBA Flex Set。CBA kit是同时检测试剂盒配好的6个指标，形式比较固定。为满足不同客户的实际需要和研究兴趣，BD于2005年初推出了CBA Flexset
Intracellular Immunofluorescent Staining for Flow Cytometry
for detection of IL-6-producing cells by human LPS-activated monocytes is 6 hours, whereas IL-10 detection needs at least 24 hours stimulation. In contrast to detection of secreted cytokines by ELISA, for detection of intracellular cytokines
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