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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.31-20ng/ml
- 检测方法:
夹心法/竞争法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
matrix metalloproteinase 9/ Gelatinase B
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.1ng/ml
- 规格:
96T
Human matrix metalloproteinase 9/ Gelatinase B ELISA Kit
Product Name:Human matrix metalloproteinase 9/ Gelatinase B ELISA Kit
Packing:96T
Catalog No.:ELA-E1315h
Gene Name:MMP-9
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:matrix metalloproteinase 9(Gelatinase B
Alternative Name:MMP-9,matrix metalloproteinase 9(Gelatinase B
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human matrix metalloproteinase 9/ Gelatinase B ELISA Kit allows for the in vitro quantitative determination of matrix metalloproteinase 9(Gelatinase B concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human matrix metalloproteinase 9/ Gelatinase B ELISA Kit has been pre-coated with an matrix metalloproteinase 9(Gelatinase B antibody specific to matrix metalloproteinase 9(Gelatinase B .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for matrix metalloproteinase 9(Gelatinase B and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain matrix metalloproteinase 9(Gelatinase B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of matrix metalloproteinase 9(Gelatinase B in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Matrix Metalloproteinase Expression in Lung Cancer
of the extracellular matrix (ECM), including components of basement membranes (BMs) and submucosa (1 ,5 ,6) . MMP-2 and MMP-9, gelatinase A and B, respectively, are of particular interest because they degrade type IV collagen, the major component of basement
kDa Gelatinase, Gelatinase A Gelatins, collagens I,IV, V, VII, X, XI, fibronectin, laminin, aggrecan, elastin, large tenascin C, vitronectin, β-amyloid protein precursor (157–159
Methods for analysis of matrix metalloproteinase regulation of neutrophil-endothelial cell adhesion
neutrophil-endothelial cell attachment. ET-1[1–32] produced by MMP-2 up-regulated CD11b/CD18 expression on human neutrophils, thereby promoted their adhesion to cultured endothelial cells. ET-1[1–32] evoked release of gelatinase B (MMP-9), which in turn
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