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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.31-20ng/ml
- 检测方法:
夹心法/竞争法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
PRAME
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.1ng/ml
- 规格:
96T
Human PRAME ELISA Kit
Product Name:Human PRAME ELISA Kit
Packing:96T
Catalog No.:ELA-E1134h
Gene Name:PRAME
Detect Range:0.156-10 ng/mL
Sensitivity:0.056ng/mL
Target Protein Name:Human Melanoma antigen preferentially expressed in tumors
Alternative Name:Melanoma antigen preferentially expressed in tumors, Opa-interacting protein 4, OIP-4, Preferentially expressed antigen of melanoma, PRAME, MAPE, OIP4
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human PRAME ELISA Kit allows for the in vitro quantitative determination of Human Melanoma antigen preferentially expressed in tumors concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human PRAME ELISA Kit has been pre-coated with an PRAME antibody specific to Human Melanoma antigen preferentially expressed in tumors .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Melanoma antigen preferentially expressed in tumors and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Melanoma antigen preferentially expressed in tumors, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm
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文献和实验Warnings And Limitations This product is for research use only. Not intended for any animal or human therapeutic or diagnostic use unless otherwise stated. Follow appropriate laboratory guidelines. This product contains 0.02% sodium azide
RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
Quantitative Analysis of PRAME for Detection of Minimal Residual Disease in Leukemia
Preferentially expressed antigen of melanoma (PRAME) was first isolated using cDNA expression cloning techniques as a gene encoding a human melanoma antigen recognized by melanoma reactive cytotoxic T-cells (CTL) (1 ). This gene codes
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