- ￥2890
- LSM Bio
- 0.31-20ng/ml
- 0.1ng/ml
- 国产
- ELA-E10999h
- 2025年07月12日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.31-20ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Divalent Metal Transporter 1
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.1ng/ml
- 规格：
96T
Human Divalent Metal Transporter 1 ELISA Kit
Product Name:Human Divalent Metal Transporter 1 ELISA Kit
Packing:96T
Catalog No.:ELA-E10999h
Gene Name:DMT1
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:Divalent Metal Transporter 1
Alternative Name:DMT1,Divalent Metal Transporter 1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Divalent Metal Transporter 1 ELISA Kit allows for the in vitro quantitative determination of Divalent Metal Transporter 1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Divalent Metal Transporter 1 ELISA Kit has been pre-coated with an Divalent Metal Transporter 1 antibody specific to Divalent Metal Transporter 1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Divalent Metal Transporter 1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Divalent Metal Transporter 1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Divalent Metal Transporter 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Resolution of Quadruplex Polymorphism by Size‐Exclusion Chromatography
. J. Biol. Chem. 271:5698‐5703. Blume, S.W., Guarcello, V., Zacharias, W., and Miller, D.M. 1997. Divalent transition metal cations counteract potassium‐induced quadruplex
Overview of Formation of G‐Quadruplex Structures
. Blume, S.W., Guarcello, V., Zacharias, W., and Miller, D.M. 1997. Divalent transition metal cations counteract potassium‐induced quadruplex assembly of oligo(dG) sequences. Nucleic Acids Res. 25: 617‐625
Analysis of Oxidative Modification of Proteins
, nitrotyrosine (when nitrogen species are the oxidants), and isoaspartate. Methods described in this unit include spectrophotometry, immunoblotting, radiolabeling, GC/MS, ELISA adapted for analysis of oxidative modification.
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