- ￥2890
- LSM Bio
- 0.15-10ng/ml
- 0.078mg/ml
- 国产
- ELA-E1005h
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.15-10ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
5- hydroxyindolacetic acid
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078mg/ml
- 规格：
96T
Human 5-Hydroxyindoleacetic acid, 5-HIAA ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
5-Hydroxyindoleacetic acid, 5-HIAA, serotonin, 5HIAA
Search name
Human 5HIAA ELSIA KIT ,Human 5-Hydroxyindoleacetic acid ELSIA KIT ,Human 5-HIAA ELSIA KIT ,Human serotonin ELSIA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Human 5-hydroxyindoleacetic acid,5-Hydroxyindoleacetic acid concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 5-Hydroxyindoleacetic acid, During the reaction, 5-Hydroxyindoleacetic acid in the sample or standard competes with a fixed amount of biotin-labeled 5-Hydroxyindoleacetic acid for sites on a pre-coated Monoclonal antibody specific to 5-Hydroxyindoleacetic acid. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of 5-Hydroxyindoleacetic acid in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PDF Manual download
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